BPF Journal Club

This Months Presenter:

Here is the article for this months discussion:
The use of aldehydes other than formaldehyde as fixatives is a comparatively recent development. In 1959 Luft demonstrated that a monoaldehyde, acrolein, could be usefully applied to solve certain problems in the preservation of fine structure. However, it was the extensive studies by Sabatini et al. (1963) that brought the usefulness of aldehydes, especially glutaraldehyde, to the attention of the electron microscopists. These studies indicated that primary fixation with glutaraldehyde followed by secondary fixation with OsO 4 yields satisfactory preservation of fine structure and enzyme activity in a wide variety of specimens. This double fixation has become the standard procedure for preserving both plant and animal specimens including prokaryotes. A mixture of glutaraldehyde and formaldehyde (freshly prepared by depolymerizing paraformaldehyde) has the additional advantage of more rapid penetration into the tissue specimen and is preferred for many specimens. Acrolein in combination with glutaraldehyde has also been employed for certain studies.
  • Michael Cerullo
    Michael CerulloM.D., Psychiatrist, Cincinnati Inst. for Cognitive Science; Author, Uploading and Branching Identity, M&M, 2014

    Leave a Comment

    Contact Us

    We're not around right now. But you can send us an email and we'll get back to you, asap.

    Not readable? Change text. captcha txt

    Start typing and press Enter to search

    Thank You for Your Support

    Please give only what you feel you can easily afford at our BPF Donation Page$5 or more is a typical online annual donation for individual donors to small nonprofits. Alternatively you may find it convenient to give using Amazon Smile, where Amazon donates 0.5% of the price of your eligible purchases to the charitable nonprofit of your choice (these donations go to the BPF General Fund). You may also donate in Bitcoin using the addresses indicated below.

    The Brain Preservation Foundation was incorporated in Delaware on August 27, 2010. We hold Section 501(c)(3) tax-exempt status as a not-for-profit scientific research organization. Your contributions are fully tax deductible. You can donate publicly or privately, as you prefer.

    Donation Options

    1. General Fund If you donate to the BPF General Fund, directors will place your donation in the category of greatest current need.

    Bitcoin: 1Q9fdbphewr5R2sLq6E98EqZu6GjeAiNys

    2. Evaluation Fund Your donation to the BPF Evaluation Fund helps us evaluate competitor submissions.

    Bitcoin: 1Daz6Euxe5yUDpQTRsTHxWci3vLC5KAzsj

    3. Technology Prize Your donation to our Technology Prize increases the size of the award.

    Bitcoin: 1HpcXrasVNtVEMyrEiUXWTjiugyKPgq535

    4. Operating Fund Your donation to the Operating Fund allows us to selectively advertise, do competitor support, and fundraise.

    Bitcoin: 1AZ8TCSLxDmxrxhEZbif927nHnoWFjNtuF

    5. Endowment Your donation here is a way to provide permanent operating support to BPF as an organization, via endowment interest. Endowment principal may not be spent as long as BPF is a legal entity. To make a major gift to the BPF endowment, please contact John Smart or Kenneth Hayworth.

    Bitcoin: 1AHR7HezVB7cRCDPE3FGEk8UKXy3ndoAv